HiTrap TALON crude

• Cat#No#: Hi-101P
• Product Overview: HiTrap TALON crude 1 mL and 5 mL columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment.
• Description: TALON Superflow is a cobalt-based IMAC resin offering different selectivity compared to nickel-charged resins. TALON Superflow and prepacked HiTrap TALON crude columns allow protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
• Characteristic: Columns prepacked with cobalt-based chromatography resins designed for purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC).
  Suitable for IMAC purification of histidine-tagged proteins when Ni2+ is not the optimal choice of metal ion.
  Compatible with commonly used IMAC reagents and appropriate for purifying proteins under native or denaturing conditions.
  Prepacked HiTrap TALON crude columns save time by allowing the application of unclarified samples directly, minimizing labor and degradation of proteins.
  HiTrap TALON crude columns allow repeated runs without stripping, cleaning, or Co2+ recharging.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Matrix: Cross-linked agarose, 6%
• Particle Size: 60-160 μm
• Dynamic binding capacity: up to 20 mg histidine-tagged protein/ ml medium.
• Recommended flow rate: 1 ml/min - 4 ml/min
• Chemical compatibility: Stable in all commonly used buffers, denaturants and detergents.
• pH working range: 2 to 14
• CIP stability: 3 to 12
• Storage: 4°C to 8°C
• Pack size: 5 × 1 mL
• Maximum flow velocity: 2000 cm/h
• Dimensions: 7 × 25 mm
• Column volume: 1 ml
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)