Butyl agarose 4 (Fast Flow) uses agarose coupled with butyl chains, high stability and pressure tolerance, optimized ligand density for highly hydrophobic biomolecules, ideal for high-flow capture or intermediate purification.
Matrix
Highly cross-linked agarose
Appearance
White paste, may separate upon standing
Average particle size
90 μm
Wet Particle Size Range
45–165 μm
Ligand
Butyl
Dynamic binding capacity
~40 μmol Ligand / mL resin
Coupling chemistry
Butyl functionalization
pH working range
3–13
pH CIP range
2–14
Chemical stability
Stable in common aqueous solutions: 1 M NaOH, 1 M acetic acid, 10 mM NaOH, 6 M guanidine HCl, 8 M urea, 70% ethanol, 30% isopropanol, 20% ethanol, 2% benzyl alcohol
Maximum operating pressure
0.3 MPa
Maximum flow velocity
≥ 150 cm/h
Pack size
25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L; 40 L
Storage
2–30 °C in 20% ethanol or 2% benzyl alcohol
Product features
Butyl ligand HIC resin; average particle size 90 μm agarose matrix; low non-specific adsorption and good flow rate; suitable for capture or medium purification of proteins with low to moderate hydrophobicity.