Butyl HIC resin uses high-rigidity agarose with low hydrophobic butyl ligands, low back pressure, fast flow, suitable for large-scale purification of highly hydrophobic biomolecules.
Matrix
High-rigidity agarose
Appearance
White paste, may separate upon standing
Wet Particle Size Range
40–120 μm
Ligand
Butyl
Dynamic binding capacity
~53 μmol Ligand / mL resin
Coupling chemistry
Butyl functionalization
pH working range
3–13
pH CIP range
2–14
Chemical stability
Stable in common aqueous solutions: 1 M NaOH, 1 M acetic acid, 6 M guanidine HCl, 30% isopropanol, 70% ethanol
Maximum operating pressure
0.5 MPa
Maximum flow velocity
≥ 1200 cm/h
Pack size
25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L; 40 L
Product features
Butyl ligand HIC resin; suitable for capture or medium purification of proteins with low to moderate hydrophobicity; high-rigidity agarose matrix with high flow rate and low back pressure; different capacity and selectivity compared to Butyl agarose FF.