Phenyl agarose FF (HS) is a high-substitution phenyl HIC medium on 6% agarose, stable and pressure-resistant, suitable for high-flow capture or intermediate purification of moderately hydrophobic biomolecules.
Matrix
Highly cross-linked agarose
Appearance
White paste, may separate upon standing
Average particle size
90 μm
Wet Particle Size Range
45–165 μm
Ligand
Phenyl
Dynamic binding capacity
~45 μmol Ligand / mL resin
Coupling chemistry
Phenyl functionalization (high substitution)
pH working range
3–13
pH CIP range
2–14
Chemical stability
Stable in common aqueous solutions: 2 M NaOH, 0.5 M NaOH, 1 M acetic acid, 0.1 M HCl, 6 M guanidine HCl, 8 M urea, 70% formic acid, 30% isopropanol, 30% acetonitrile, 2% SDS, 3 M (NH4)2SO4, 0.1 M KH2PO4 (pH 7.0), 24% ethanol
Maximum operating pressure
0.3 MPa
Pack size
25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L; 40 L
Storage
2–30 °C in 20% ethanol or 2% benzyl alcohol
Product features
High-density phenyl ligand HIC resin; average particle size 90 μm agarose matrix; good non-specific adsorption and flow rate; strongly hydrophobic ligand, suitable for capture or medium purification of weakly hydrophobic recombinant proteins, antibodies, enzymes, and nucleic acids.