Phenyl agarose (Fast Flow, Low Sub) is a low-substitution phenyl HIC medium on agarose, with high physical and chemical stability, ideal for high-flow purification of moderately hydrophobic biomolecules in capture or intermediate purification.
Matrix
Highly cross-linked agarose
Appearance
White paste, may separate upon standing
Average particle size
90 μm
Wet Particle Size Range
45–165 μm
Ligand
Phenyl
Dynamic binding capacity
~25 μmol Ligand / mL resin
Coupling chemistry
Phenyl functionalization (low substitution)
pH working range
3–13
pH CIP range
2–14
Chemical stability
Stable in common aqueous solutions: 1 M NaOH, 1 M acetic acid, 6 M guanidine HCl, 30% isopropanol, 70% ethanol
Maximum operating pressure
0.3 MPa
Maximum flow velocity
250–400 cm/h
Pack size
25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L; 40 L
Storage
2–30 °C in 20% ethanol or 2% benzyl alcohol
Product features
Low-density phenyl ligand HIC resin; different selectivity compared to Phenyl agarose FF (HS); average particle size 90 μm agarose matrix; good non-specific adsorption and flow rate performance; strongly hydrophobic ligand, suitable for capture or medium purification of weakly hydrophobic recombinant proteins, antibodies, enzymes, and nucleic acids.