High-flow phenyl HIC resin uses fine high-rigidity agarose with moderately hydrophobic phenyl ligands, high resolution, low back pressure, suitable for intermediate and fine protein purification.
Matrix
Highly cross-linked agarose
Appearance
White paste, may separate upon standing
Wet Particle Size Range
36–44 μm
Ligand
Phenyl
Dynamic binding capacity
~19 mg BSA / mL packed resin
pH working range
3–13
pH CIP range
2–14
Chemical stability
Stable in common aqueous solutions: 2 M NaOH, 0.5 M NaOH, 1 M acetic acid, 6 M guanidine HCl, 8 M urea, 30% isopropanol, 30% acetonitrile, 2% SDS, 3 M (NH4)2SO4 + 0.1 M KH2PO4 (pH 7.0)
Maximum operating pressure
0.5 MPa
Maximum flow velocity
≥ 450 cm/h
Recommended flow rate
90–180 cm/h
Pack size
25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L; 40 L
Product features
Phenyl ligand HIC resin; high-rigidity, high-resolution agarose matrix; high throughput with higher resolution; strongly hydrophobic ligand, suitable for medium or fine purification of weakly hydrophobic recombinant proteins, antibodies, enzymes, and nucleic acids.