Hydrophobic interaction chromatography (HIC) separates biomolecules via interactions between hydrophobic groups on the molecule and hydrophobic ligands on the stationary phase. Salt ions disrupt hydration shells, promoting binding. high-substitution phenyl HIC resin uses high-rigidity agarose with highly substituted phenyl ligands, offering low back pressure and fast flow rates, suitable for large-scale purification.
Matrix
High-rigidity agarose
Appearance
White paste, may separate upon standing
Wet Particle Size Range
40–120 μm
Ligand
Phenyl
Dynamic binding capacity
~27 mg BSA / mL packed resin
Coupling chemistry
Phenyl functionalization (high substitution)
pH working range
3–13
pH CIP range
2–14
Chemical stability
Stable in common aqueous solutions: 1 M NaOH, 1 M acetic acid, 6 M guanidine HCl, 30% isopropanol, 70% ethanol
Maximum operating pressure
0.5 MPa
Maximum flow velocity
≥ 1200 cm/h
Pack size
25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L; 40 L
Product features
High-density phenyl ligand hydrophobic interaction chromatography (HIC) resin; high-rigidity agarose matrix with high flow rate and low back pressure; compared to Phenyl agarose FF, offers different capacity and selectivity; strongly hydrophobic ligand, suitable for capture or medium purification of weakly hydrophobic recombinant proteins, antibodies, enzymes, and nucleic acids.
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For Research or Industrial Raw Materials, Not For Personal Medical Use!