Low-substitution phenyl HIC resin is a HIC medium with high-rigidity agarose and low-substitution phenyl ligands, low back pressure, fast flow, ideal for large-scale biomolecule purification.
Matrix
High-rigidity agarose
Appearance
White paste, may separate upon standing
Wet Particle Size Range
40–120 μm
Ligand
Phenyl
Dynamic binding capacity
~22 mg BSA / mL packed resin
Coupling chemistry
Phenyl functionalization (low substitution)
pH working range
3–13
pH CIP range
2–14
Chemical stability
Stable in common aqueous solutions: 1 M NaOH, 1 M acetic acid, 6 M guanidine HCl, 30% isopropanol, 70% ethanol
Maximum operating pressure
0.5 MPa
Maximum flow velocity
≥ 1200 cm/h
Pack size
25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L; 40 L
Product features
Low-density phenyl ligand HIC resin; high-rigidity agarose matrix with high flow rate and low back pressure; compared to Phenyl agarose FF, offers different capacity and selectivity; strongly hydrophobic ligand, suitable for capture or medium purification of weakly hydrophobic recombinant proteins, antibodies, enzymes, and nucleic acids.