IEX-SP Agarose 6 Fast Flow

• Cat#No#: IE-004D
• Product Overview: Ion Exchange Media CM, SP, DEAE, Q Agarose 6FF are specifically developed for high-resolution separation of proteins, peptides, nucleic acids and other charged biomolecules according to their difference in surface charge. The base matrix of IEX-CM, IEX-SP, IEX-DEAE, IEX-Q is highly cross-linked 6% agarose, providing excellent chemical and physical stability.
  The type of charged group determines the type and strength of the exchanger, while the total number and availability of the charged groups determine the capacity. Sulfonic and quaternary amines form strong ion exchangers, which are completely ionized over a broad pH range. Others form weak ion exchangers, where the degree of dissociation, and thus the exchange capacity, varies markedly with pH. "Strong" and "weak" refer to the extent of ionization with pH, and not to the strength of binding.
  Our ion exchanger media are optimized for high sample loading capacity with reliable, reproducible performance, suited for process scale chromatography.
• Matrix: Highly cross-linked 6% agarose supplied as a 50% slurry
• Ionic Exchanger Type: Strong cation
• Ion exchange capacity: 0.18 – 0.25 mmol H+/mL medium
• Average particle size: 45 μm - 165 μm
• Recommended flow rate: 400-700 cm/h
• pH working range: pH 4 - 13
• Storage: 2 - 30°C, storage buffer: 20% ethanol, 0.2 M Sodium acetate