IgM Capture Resin

• Product Overview: This affinity medium is prepared by immobilizing the sulfur-containing compound 2-mercaptopyridine onto fine, high-rigidity agarose beads, creating a thiophilic resin. The optimized ligand density provides appropriate affinity for IgM, and the fine beads increase the binding capacity for large IgM molecules. Thiophilic interactions exploit electron donor-acceptor forces to separate and purify biomolecules, which are strengthened in high-salt conditions and weakened in low-salt environments.
• Matrix: 6% Highly cross-linked agarose
• Appearance: White paste, may separate upon standing
• Wet Particle Size Range: 36–44 μm
• Ligand: 2-Mercaptopyridine
• Dynamic binding capacity: ~5 mg Human IgM / mL resin
• Coupling chemistry: Epoxy chemistry
• pH working range: 3–11
• pH CIP range: 2–13
• Chemical stability: Stable in common aqueous solutions: 70% ethanol, 30% isopropanol, 1 M acetic acid, 0.1 M NaOH
• Maximum operating pressure: 0.5 MPa
• Pack size: 25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L
• Storage: 2–30 °C in 20% ethanol or 2% benzyl alcohol
• Product features: Thiol-specific affinity resin with 2-mercaptopyridine ligand.
  High Resolution and Binding Capacity: Efficient elution with high product recovery and activity retention.