IMAC Resin (Fast Flow)

• Product Overview: This metal-chelating affinity medium is widely used for purifying proteins and peptides. Its principle relies on interactions between transition metal ions (Cu²⁺, Zn²⁺, Ni²⁺, Co²⁺, Fe³⁺) immobilized on the resin and the histidine, cysteine, or tryptophan side chains of target proteins.
• Matrix: 6% Highly cross-linked agarose
• Appearance: White paste, may separate upon standing
• Wet Particle Size Range: 45–165 μm
• Ligand: Iminodiacetic acid (IDA)
• Dynamic binding capacity: Ni²⁺: ~40 mg His-tagged protein / mL packed resin
• Coupling chemistry: Metal chelation (iminodiacetic acid)
• pH working range: 3–12
• pH CIP range: 2–14
• Chemical stability: 40 °C 1 week: 10 mM HCl, 0.1 M NaOH, 8 M urea, 6 M guanidine HCl
• Maximum operating pressure: 0.3 MPa
• Maximum flow velocity: ~600 cm/h
• Pack size: 25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L
• Storage: 2–30 °C in 20% ethanol or 2% benzyl alcohol
• Product features: Non-precharged metal ion resin capable of chelating Cu²⁺, Zn²⁺, Ni²⁺, Co²⁺, etc.
  High stability and specificity, fast flow rate, and high resolution.