IMAC Sepharose 6 Fast Flow resin

• Cat#No#: IM-369C
• Product Overview: IMAC Sepharose 6 Fast Flow is an uncharged IMAC resin for purifying proteins and peptides with affinity for metal ions in batch mode or when scale up is needed:
  Supplied uncharged, for customized metal ion charging and optimized selectivity.
  Suitable for purification of histidine-tagged (his-tagged) proteins when nickel is not the best choice of metal ion.
  Based on well-established Sepharose Fast Flow resins. High chemical stability enables proven CIP and sanitization protocols. Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
• Description: IMAC Sepharose 6 Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.
• Characteristic: Convenient purification of histidine-tagged proteins when Ni²+ is not the best choice of metal ion.
  Charge with your metal ion of choice to optimize selectivity.
  High binding capacity.
  BioProcess medium designed to meet manufacturing needs for security-of-supply, robust performance, and regulatory support.
  Available in prepacked HiTrap columns for convenient purification and prepacked HiPrep columns for easy scale-up.
• Maximum operating pressure: 1 bar (14.5 psi, 0.1 MPa) (when packed in XK columns; may vary if used in other columns)
• Sample preparation: (Histidine)6 -tagged proteins: Capacity data were obtained for a protein (Mr 28 000) bound from an E. coli extract, and a pure protein (Mr 43 000; applied at 1 mg/ml in binding buffer; capacity at 10% breakthrough).
  Untagged protein: Capacities determined at 10% breakthrough for human apotransferrin applied at 1 mg/ml in binding buffer.
• Metal ion capacity: ~25 µmol Cu²₊/ml medium, ~15 µmol Ni²₊ or Zn²₊/ml medium
• Packing Column: IMAC Sepharose 6 Fast Flow is supplied preswollen in 20% ethanol. Prepare a slurry by decanting the 20% ethanol solution and replacing it with distilled water in a ratio of 75% settled medium to 25% distilled water.
• Matrix: 6% cross-linked agarose
• Particle Size: 45 μm-165 μm
• Average particle size: ~90 µm
• Dynamic binding capacity: ~40 mg histidine-tagged protein/mL resin when changed with Ni²
• Recommended flow rate: 150 cm/h
• Chemical stability: Stable in commonly used aqueous buffers - 0.01 M HCl, 1 M NaOH, 70% acetic acid, 2% SDS. 30% 2-propanol
• pH working range: 3–12
• CIP stability: 3–12
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: In some applications, substances such as denatured proteins or lipids are not removed during the regeneration procedures. These substances can be removed by Cleaning-In-Place (CIP). The column should be cleaned when the back pressure increases, or to avoid cross-contamination between samples/target proteins.
• Pack size: 25 mL
• BioProcess resin: Yes
• Maximum flow velocity: 600 cm/h (20 mL/min) using XK 16/20 columns with 5 cm bed height
• Column volume: 0.25 ml or 1 ml.
• Column hardware pressure limit: 5 bar (0.5 MPa, 72 psi)