KappaSelect

• Cat#No#: Ka-327C
• Product Overview: Antibody fragments, especially Fabs, are getting increased attention as potential biopharmaceuticals because they have some advantages over monoclonal antibodies (MAbs), KappaSelect is an affinity resin designed for the purification of human Fab (kappa) fragments.
• Description: Antibody fragments are gaining increased attention as potential biopharmaceuticals because they display certain advantages over monoclonal antibodies (MAbs).
  KappaSelect is affinity chromatography resins for purifying kappa and lambda Fab fragments, respectively. These resins enable efficient capture with high purity and yield.
• Optimum pH: 12
• Characteristic: Efficient, industrial-scale capture of Fabs by affinity chromatography.
  High binding capacity for Fabs.
  Rigid agarose base matrix allows high flow rates and processing of large sample volumes for increased throughput.
  Non-mammalian derived product reduces regulatory concerns in the production of Fabs for clinical applications.
  Low ligand leakage ensures increased Fab purity and productivity.
• Applications: KappaSelect is an affinity chromatography resin designed for the purification of Fab (kappa) with high binding capacity, purity and yield.
• Maximum operating pressure: 300 kPa at 600 cm/h
• Ligand Coupling Method: Amide linkage
• Packing Column: Pack the column with resin.
• Column: 0.4 mL LambdaFabSelect packed in a Tricorn 5/20 column.
• Matrix: Highly cross-linked agarose
• Average particle size: ~75 µm
• Ligand: Recombinant protein (Mr 13 000), produced in S. cerevisiae, with affinity for the constant domain of the immunoglobulin kappa light chain.
• Ligand density: Approx. 5 mg/ml of medium.
• Dynamic binding capacity: Approximately 15 mg Fab/mL of resin
• Recommended flow rate: At least 600 cm/h in a 1 m diameter column, with 20 cm bed height at 20°C using buffers with the same viscosity as water at < 0.3 MPa (3 bar).
• Chemical stability: Stable to commonly used aqueous buffers: 6 M urea, 6 M guanidine hydrochloride, 70 % ethanol, 30 % isopropanol.
• Physical stability: The ligand is immobilized to the agarose base matrix via stable amide bonds that ensure high chemical stability and low leakage.
• pH working range: 3–10
• pH CIP range: 2–12
• CIP stability: KappaSelect showed good stability up to pH 12. Use of a low pH solution or agents like guanidine hydrochloride in a cleaning protocol is therefore recommended for KappaSelect. For KappaSelect, avoid pH > 12 due to limited ligand stability under strongly alkaline conditions.
• Temperature stability: 4°C to 30°C
• Storage: 2 to 8°C, 20% Ethanol
• Cleaning-in-place: The recommended protocol comprises initial strip of the resin at low pH, and then subjecting the resin to NaOH of low concentration for cleaning.
• Sanitization: PAB (120 mM phosphoric acid, 167 mM acetic acid, 2.2 % v/v benzyl alcohol) is used for final sanitization of the resin. PAB solution is sensitive to light and should be freshly made not to damage the resin. PAB solution should be stored in a dark bottle and kept no longer than for a week. PAB solution has a pH of < 2, and resin stability can be limited in prolonged exposure at such a low pH.
• Pack size: 25 mL
• Wash buffer: Phosphate buffered saline (PBS), pH 7.4 (0.01 M phosphate buffer, 0.0027 M KCl, 0.14 M NaCl).