Large Pore Size 300nm DEAE PS-DVB Resin

• Alias: LPS; DEAE; Ion Exchange
• productkeywords: LPS; Large Pore Size 300nm DEAE PS-DVB Resin; LPS-50V-DEAE2
• Product Overview: Large Pore Size 50V DEAE super porous weak anion exchange chromatography medium, using polymer PS-DVB (polystyrene - divinylbenzene copolymer) as the matrix, the matrix is made of sugar-containing polymer and polystyrene composite, high mechanical strength, good chemical stability, the surface through a unique hydrophilic modification, It has low non-specific adsorption and can withstand high salt loading. The product has the advantages of high flow rate, high load capacity, high alkali resistance and low back pressure through the optimization of medium substrate and ligand density, as well as the design of open super porous structure. The weak anion exchange (DEAE) chromatography medium was obtained by bonding ion exchange functional groups on the surface of hydrophilic medium with a special surface modification technique.
• Product characteristics: High loading capacity: 3000Å large aperture can make more virus particles enter the packing hole for exchange and combination;
  High yield: It can maintain the structural integrity of macromolecular proteins or viruses, and obtain the target proteins with high yield and high activity;
  High column efficiency: molecular mass transfer speed is fast, protein or virus particles can easily enter the hole to bind;
  High salt tolerance: high salt loading can be tolerated, eliminating the desalting step, simplifying the operation process and reducing costs;
  Flow rate: Fast flow rate, suitable for processing large volume samples in a short time, improve production efficiency.
• Particle size: 50μm
• aperture: 3000Å (300nm)
• Ligand density: 0.12 meq/mL
• Load per milliliter: >65mg BSA
• Recommended flow rate: 150~600cm/h
• pH: 2~12(work); 1~13(CIP)
• Form: 20% ethanol.
• Applications: Isolation and purification of AAV, VLP particles, viruses, herpes simplex virus vaccine, etc.
• Chemical stability: 1M acetic acid, 1M sodium hydroxide, 1M hydrochloric acid, 70% ethanol, 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants.
• Storage: Store at 2~30°C, with 20% ethanol.
• Matrix: PS-DVB
• Ligand: -N+(C2H5)2
• Maximum withstand pressure: 3 Mpa