Lens Culinaris Agglutinin, abbreviated as LCA, is a kind of metallic protein isolated from the seeds of lentils. It is composed of two large subunits of 17 kD and two small subunits of 8 kD, with a molecular weight of approximately 49 kDa. It can specifically bind to molecules containing α -D-mannose, α -D-glucose or spatially related residues. Beaver LCA magnetic beads are a new type of affinity magnetic beads that couple lentil lectins with agarose and utilize the specific reversibility of mutual recognition between lectins and glycoprotein sugar chains to separate and purify glycoproteins, and are used for enriching or purifying glycoproteins. Magnetic separation methods do not require complex operations such as centrifugation, filtration, and chromatography. They only need to be applied with an external magnetic field to separate the target protein, eliminating numerous sample pretreatment and separation processes. Compared with traditional separation and purification methods, magnetic separation has the advantages of rapidity, high efficiency and high purity.
Particle size
30-150 μm
Binding capacity
≥13 mg thyroglobulin /mL medium
Suspension concentration
10%(v/v)magnetic beads suspension
Matrix
Highly cross-linked 4% agarose
Ligand
Lentil lectin
Applications
It is suitable for the separation and purification of some glycoproteins, membrane proteins, glycolipids, polysaccharides, membrane vesicles with mannoside or glucoside residues, IgM, hormone lipoproteins, etc.