Lentil Lectin Sepharose 4B resin for glycoprotein purification

• Cat#No#: Le-373C
• Product Overview: Lentil Lectin Sepharose 4B resin is used for glycoprotein purification and purification of other carbohydrate-containing molecules. Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related sugars.
  Compared to Con A, lentil lectin distinguishes less sharply between glucosyl and mannosyl residues.
  Retains binding in 1% deoxycholate and is valuable for purifying detergent-solubilized membrane proteins.
• Description: Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars. Lentil Lectin Sepharose 4B is a generally applicable group specific adsorbent and is routinely used in the preparation and purification of a number of glycoproteins and carbohydrate containing molecules. Detergent-solubilized membrane glycoproteins, cell surface antigens viral glycoproteins may also be purified using Lentil Lectin Sepharose 4B.
• Applications: For purification, isolation or removal of molecules containing branched mannoses with fucose linked α(1,6) to the N-acetylglucosamine, (αMan>αGlc>GlcNAc) and sterically related residues like glycoproteins, membrane proteins, glycolipids, lipoproteins, pol.
• Maximum operating pressure: Base matrix 70-140 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.
• Medium Preparation: Lentil Lectin Sepharose 4B is supplied preswollen in 20% ethanol containing 0.9% NaCl, 1 mM CaCl2 and 1 mM MnCl2 . Wash the required amount of medium with 10 volumes of binding buffer to remove the ethanol solution. Prepare a slurry with binding buffer in a ratio of 75% settled medium to 25% buffer.
  The binding buffer should not contain agents which significantly increase the viscosity. The column may be equilibrated with viscous buffers at reduced flow rates after packing is completed.
• Ligand Coupling Method: Cyanogen bromide activation
• Matrix: Agarose, 4%
• Particle Size: 45 µm-165 µm
• Average particle size: ~90 µm
• Ligand: Lentil lectin
• Ligand density: 2 mg Lentil lectin/ml medium
• Recommended flow rate: < 75 cm/h at 25 °C, HR 16/10 column, 5 cm bed height
• Recommended column height: 25 cm
• Chemical stability: Stable to commonly used aqueous buffers. Chelating agents such as EDTA, 8 M urea or solutions having a pH below 3 should be avoided as these conditions result in removal of manganese from the lectin and loss of activity.
• Physical stability: Negligible volume variation due to changes in pH or ionic strength.
• pH working range: 3–10
• CIP stability: 3–10
• Storage: 2 to 8°C, 20% Ethanol, 0.9% NaCl, 1 mM Ca2+ and 1 mM Mn2+
• Pack size: 25 mL