Lys N Protease

• sizes: 20 μg
• Description: Lys-N is an innovative protease that specifically hydrolyzes peptide bonds at the N-terminus of lysine residues. This enzyme is applicable for post-translational modification (PTM) research and produces more b-ions than y-ions in the mass spectrometer. Combining Lys-N with rTrypsin-N provides an optimal strategy for protein sequencing.
• Physical Appearance: Lyophilized powder
• Molecular Weight: 18.4 kDa
• Resuspension Buffer: 50 μM Zinc Acetate buffer or Zinc Sulfate buffer
• Storage: Store the lyophilized powder at –20°C. Store reconstituted enzyme at -80 °C for up to 30 days.
• Shelf life: 24 months at -80 ℃.
• Stability: Maximally active in the pH range 7 - 9.
• Usage Notes: Resuspend 20 μg of Mass Spectrum Grade Lys-N in 40 μL resuspension buffer for maximum activity. Add 50 mM ammonium bicarbonate or Tris-HCl (pH 8) to the protein mixture (recommended). Add 0.5 μg/μL Lys-N to reach a final enzyme-to-substrate ratio of 1:30 for digestion. Mix well and incubate at 37 °C for 4 hours.
• Activity: 394 U/mg