Lysine Sepharose 4B

• Cat#No#: Ly-374C
• Product Overview: Lysine Sepharose 4B resin with immobilized amino acid lysine is mainly used for isolation of plasminogen and plasminogen activator. The ε-amino and α-carboxyl groups on lysine is free after immobilization and interact with a range of biomolecules.
  Mainly used for isolation of plasminogen and plasminogen activator, separation of ribosomal RNA (rRNA) and purification of doublestranded DNA.
  It is designed for purification of molecules with biospecific or charge dependent affinity for L-lysine.
• Description: Lysine Sepharose 4B is L-lysine immobilized to Sepharose 4B by the cyanogen bromide method. L-lysine is coupled via its α-amino group, leaving both the ε-amino and α-carboxyl groups free to interact with sample substances during chromatography. It is designed for purification of molecules with biospecific or charge dependent affinity for L-lysine. Lysine Sepharose 4B is a group specific adsorbent and has been used for isolation of plasminogen and plasminogen activator, separation of ribosomal RNA (rRNA) and purification of doublestranded DNA.
• Ligand Coupling Method: Cyanogen bromide activation
• Matrix: Agarose, 4%
• Particle Size: 45 µm-165 µm
• Average particle size: ~90 µm
• Ligand: L-lysine
• Recommended flow rate: < 75 cm/h at 25 °C, HR 16/10 column, 5 cm bed height
• Chemical stability: Stable to commonly used aqueous buffers.
• pH working range: 2–11
• CIP stability: 2–11
• Storage: 2 to 8°C
• Pack size: 15 g