MabAffinity rProtein A Agarose High Flow

• Common Name: rProtein A
• Cat#No#: MA-003A
• Product Overview: Mab Affinity Protein A Agarose High Flow is a high capacity, chemically stableaffinity medium designed specially for the purification of monoclonal and polyclonal antibodies at laboratory and process scales.
  
• Form: Slurry in 20% Ethanol
• Characteristic: Recombinant protein A has a molecular weight of Mr 44 600 and contains five antigen binding domains (E, D, A, B, C) which have strong and roughly equal, affinity for the Fc region of immunoglobulin. One molecule of protein A can bind approximately two molecules of IgG.
  
• Storage: Unused media can be stored in the container at a temperature of +4 to +8°C. Ensure that the screw top is fully tightened.
  Packed columns should be equilibrated in binding buffer containing 22% ethanol to prevent microbial growth.
  After storage, equilibrate with at least 5 bed volumes of starting buffer before use.
• Packing Column: Mab Affinity Protein A Agarose High Flow is supplied as suspension in 20% ethanol. Decant the 20% ethanol solution and replace it with packing buffer before use.
  
• Matrix: Highly cross-linked Agarose
• Molecular Characterization: Recombinant protein A has a molecular weight of Mr 44 600
• Average particle size: 90 μm
• Ligand: rProtein A
• Coupling chemistry: Epoxy
• Dynamic binding capacity: > 30mg IgG /ml media
• Recommended flow rate: 100-300cm/h
• Viscosity: Unused media can be stored in the container at a temperature of +4 to +8°C. Ensure that the screw top is fully tightened.
  Packed columns should be equilibrated in binding buffer containing 22% ethanol to prevent microbial growth.
  After storage, equilibrate with at least 5 bed volumes of starting buffer before use.
• Recommended column height: 5‐20cm
• Maximum Pressure Drop: 3 bar
• Chemical stability: Stable in all aqueous buffers commonly used in protein A chromatography
• pH working range: 3-9
• pH CIP range: 2-11
• CIP stability: 8M Urea, 6M Gua‐HCl or 1mM NaOH+1M
  NaCl pH 11, 0.1M acetic acid
• Temperature stability: 2‐40 °C
• Shelf life: 5 years
• Warning: 1. Determined at 10% breakthrough at 4min residence time
  2. Delivered at room temperature, and recommended long‐term storage at 2‐8 °C