MabAffinity rProtein G Agarose High Flow

• Common Name: rProtein G
• Cat#No#: MG-001A
• Product Overview: Mab Affinity Protein G Agarose High Flow Resin is a high capacity, chemically stable affinity medium designed specially for the purification of monoclonal and polyclonal antibodies and isolation of immune complex at laboratory and process scales.
  
• Form: Slurry in 20% Ethanol
• Characteristic: Recombinant Protein G has a mass of 21.6kDa,while its apparent size is 31 to 34 kDa by SDS-PAGE and contains only IgG binding domains. Compared to Protein A, Protein G binds more efficiently to polyclonal IgG from cow, sheep and horse, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike Protein A, Protein G does not bind to human IgM, IgD and IgA. 1 ML of protein G-agarose binds 10 to 20 mg of pure IgG, which depends on the species and the IgG subclass.
  
• Storage: Unused media can be stored in the container at a temperature of +4 to +8°C. Ensure that the screw top is fully tightened and keep container tightly closed in a dry and well-entilated place.
  Packed columns should be equilibrated in binding buffer containing 20% ethanol to prevent microbial growth.
  After storage, equilibrate with at least 5 bed volumes of starting buffer before use.
  
• Packing Column: Mab Affinity Protein G Agarose High Flow is supplied as suspension in 20% ethanol. Decant the 20% ethanol solution and replace it with packing buffer or water before use.
  
• Matrix: Highly cross-linked Agarose
• Molecular Characterization: Recombinant Protein G has a mass of 21.6kDa,while its apparent size is 31 to 34 kDa by SDS-PAGE
• Average particle size: 90 μm
• Ligand: rProtein G
• Coupling chemistry: Epoxy
• Dynamic binding capacity: 18 mg IgG /ml media
• Recommended flow rate: 100-300cm/h
• Viscosity: Unused media can be stored in the container at a temperature of +4 to +8°C. Ensure that the screw top is fully tightened and keep container tightly closed in a dry and well-entilated place.
  Packed columns should be equilibrated in binding buffer containing 20% ethanol to prevent microbial growth.
  After storage, equilibrate with at least 5 bed volumes of starting buffer before use.
  
• Recommended column height: 5‐20cm
• Maximum Pressure Drop: 3 bar
• Chemical stability: Stable in all aqueous buffers commonly used in protein G chromatography
• pH working range: 2-9
• pH CIP range: 2-10
• CIP stability: 8M Urea, 6M Gua‐HCl, 3 M sodium
  isothiocyanate, 0.1M acetic acid
• Temperature stability: 2‐40 °C
• Shelf life: 5 years
• Warning: 1. Determined at 10% breakthrough at 4min residence time
  2. Delivered at room temperature, and recommended long‐term storage at 2‐8 °C