MabSelect antibody purification chromatography resin

• Cat#No#: Ma-334C
• Product Overview: MabSelect is a BioProcess protein A resin for capturing monoclonal antibodies (mAbs) from large sample volumes.
  High-flow agarose matrix allows for high flow velocities at production scale enabling the processing of more than 10 000 L of feed in one working day.
  Oriented coupling of the ligand and optimized matrix gives high dynamic binding capacity, which reduces resin volume requirements.
  Low non-specific binding resulting in low levels of impurities in the product eluate pool
  BioProcess resin supported for industrial applications and well-established in approved processes.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Description: MabSelect is a high-throughput Protein A chromatography resin that has been designed into several new and second-generation processes for the production of therapeutic monoclonal antibodies (mAbs). The hydrophilic, high-flow agarose particle, optimized for both capacity and throughput, and the oriented coupling of the rProtein A ligand, deliver a product pool that is high in purity and yield. The rProtein A ligand is expressed in E. coli and is free of components of mammalian origin.
• Characteristic: MabSelect is a member of the MabSelect product line of rProtein A-based affinity resins for capturing monoclonal antibodies. Like its companion products, MabSelect is based on an innovative, high-flow agarose base matrix platform, optimized for maximum capacity. The rProtein A is coupled to the base matrix at the C-terminal cysteine via a stable thio-ether bond. This oriented coupling contributes to the increased capacity seen with this resin.
• Ligand Coupling Method: Epoxy activation
• Matrix: Highly cross-linked agarose, spherical
• Average particle size: ~85 µm
• Ligand: Recombinant protein A (E.coli)
• Coupling chemistry: Epoxy
• Dynamic binding capacity: ~30 mg hlgG/mL resin
• Recommended flow rate: 500 cm/h
• Chemical stability: Stable in aqueous buffers commonly used in Protein A Chromatography; 10 mM NaOH (pH 12), 0.1 M 20% sodium citrate/HCl (pH 3), 6M Gua-HCl, 8M urea, 20% ethanol, 2% benzyl alcohol.
• pH working range: 3–10
• CIP stability: 3–12
• Temperature stability: 2°C to 40°C
• Storage: 2 to 8°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol).
• Shipping: 20% ethanol
• Cleaning-in-place: Wash with 2 column volumes of 50 mM NaOH. Contact time for CIP must be at least 10 minutes. Wash immediately with at least 5 column volumes of sterile filtered binding buffer at pH 7 to 8. Reversed flow direction.
• Sanitization: Sanitization reduces microbial contamination of the bed to a minimum. Equilibrate the column with a solution consisting of 2% hibitane digluconate and 20% ethanol. Allow to stand for 6 hours, then wash with at least 5 column volumes of sterile binding buffer. Equilibrate the column with a solution consisting of 0.1 M acetic acid and 20% ethanol. Allow to stand for 1 hour, then wash with at least 5 column volumes of sterile binding buffer. Equilibrate the column with 70% ethanol. Allow to stand for 12 hours, then wash with at least 5 column volumes of sterile binding buffer.
• Pack size: 25 mL