MabSelect PrismA protein A chromatography resin

• Cat#No#: Ma-329C
• Product Overview: MabSelect PrismA affinity chromatography resin has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein A-derived ligand. This allows future demands in mAb purification to be met, including processing of many bispecific antibodies.
  Enhanced dynamic binding capacity allows high mass throughput of processed mAb per resin volume unit.
  Excellent alkaline stability enables efficient cleaning and sanitization using 0.5–1.0 M NaOH for improved process economy, bioburden control, and robustness.
  Covered by a comprehensive security of supply program, including dual sources of the agarose base matrix and protein A ligand.
  The PrismA protein A ligand is also available in HiTrap Fibro and HiScreen Fibro PrismA chromatography units for ultrafast purification of mAbs.
• Description: MabSelect PrismA is a next-generation Protein A chromatography resin that offers significantly enhanced alkaline stability and binding capacity for improved process economy in monoclonal antibody (mAb) processing. The resin builds on the proven track record of MabSelect and MabSelect SuRe resins in commercial mAb production. In comparison with its predecessors, however, MabSelect PrismA has been improved with an optimized highflow agarose base matrix and a genetically engineered Protein A-derived ligand, allowing future demands in mAb processing to be met.
• Characteristic: Enhanced dynamic binding capacity (DBC) allows high mass throughput of processed mAb per resin volume unit.
  Excellent alkaline stability enables efficient cleaning and sanitization using 0.5–1.0 M NaOH for improved process economy and robustness.
  Covered by a comprehensive security of supply program, including dual sources of the agarose base matrix and Protein A ligand.
• Ligand Coupling Method: Epoxy
• Matrix: Highly cross-linked agarose, spherical.
• Average particle size: 60 µm
• Ligand: Alkaline-stabilized protein A-derived (E.coli).
• Coupling chemistry: Epoxy
• Dynamic binding capacity: 80 mg human IgG/mL resin at 6 min residence time. 65 mg human IgG/mL resin at 4 min residence time.
• Chemical stability: Stable to commonly used aqueous buffers in protein A chromatography.
• pH working range: 3–12
• pH CIP range: 2–14
• Storage: 2˚C to 8˚C, 20% ethanol
• Shipping: 20% ethanol;On request 2% benzyl alcohol (BnOH).
• Cleaning-in-place: 1.Wash the column with 3 column volumes (CV) of binding buffer.
  2.Wash with at least 3 CV NaOH (0.5 to 1.0 M ), with a contact time of 15 minutes.
  3.Wash immediately with at least 5 CV sterile and filtered binding buffer at pH 7 to 8.
• Sanitization: Sanitization reduces microbial contamination of the chromatographic bed to a minimum. MabSelect PrismA is alkali-tolerant allowing the use of NaOH as sanitizing agent. NaOH is very effective for inactivating viruses, bacteria, yeasts, and endotoxins. In addition, NaOH is inexpensive compared with other sanitizing agents.
• Pack size: 25 mL
• Maximum flow velocity: 300 cm/h