MabSelect SuRe antibody purification resin

• Cat#No#: Ma-332C
• Product Overview: The recombinant protein A ligand of MabSelect SuRe has been engineered to withstand 0.1–0.5 M NaOH, enabling efficient cleaning of the chromatography resin.
  In process-scale applications, this NaOH tolerance extends the lifetime of the resin and allows for the use of rigorous and cost-effective CIP and sanitization protocols.
  In research applications, this NaOH tolerance enables the purification of multiple antibody types with minimized cross-contamination risk.
  Protein ligand designed for enhanced protease resistance compared with unmodified protein A leads to lower ligand leakage.
  Meets industrial demands in terms of comprehensive technical and regulatory support and security of supply.
  Animal-component free raw materials and manufacturing process.
• Description: MabSelect SuRe (Superior Resistance) is a member of the MabSelect family of affinity chromatography resins for the capture of monoclonal antibodies (mAbs) at process scale. MabSelect SuRe is composed of a rigid, high-flow agarose matrix and alkali-stabilized protein A-derived ligand. This ligand provides greater stability than conventional protein A-based resins under the alkaline conditions used in cleaning-in-place (CIP) protocols. The enhanced alkali stability of MabSelect SuRe improves process economy; cleaning can be performed with cost-effective reagents such as sodium hydroxide, which improves process economy and product quality.
• Characteristic: Novel, alkali-stabilized protein A ligand allows the use of 0.1–0.5 M sodium hydroxide for CI.
  Improves product quality and reduces overall costs.
  Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
  Generic elution conditions for different monoclonal antibodies enables platform approach to purification.
  High dynamic binding capacity (DBC) reduces process time and amount of resin used.
  High-flow agarose matrix allows processing of large volumes of feed.
• Sample preparation: 8 mg humanized IgG1 in HCCF/5 mg humanized IgG4 in HCCF
• Ligand Coupling Method: Epoxy activation
• Column: Tricorn 5/100
• Matrix: Highly cross-linked agarose, spherical
• Average particle size: ~85 µm
• Ligand: Alkali-stabilized Protein A-derived (E. coli)
• Coupling chemistry: Epoxy
• Dynamic binding capacity: ~35 mg hIgG/mL resin
• Recommended flow rate: 100 to 500 cm/h
• Chemical stability: Stable in aqueous buffers commonly used in Protein A Chromatography.
• pH working range: 3–12
• pH CIP range: 2–13.7
• CIP stability: 0.1 to 0.5 M NaOH
• Temperature stability: 2°C to 40°C
• Storage: 2 to 8°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol).
• Shipping: 20% ethanol
• Cleaning-in-place: 1.Wash the column with 3 column volumes of binding buffer.
  2.Wash with at least 2 column volumes of 0.1 to 0.5 M NaOH. Contact time 10 to 15 minutes.
  3.Wash immediately with at least 5 column volumes of sterile and filtered binding buffer at pH 7 to 8.
• Sanitization: Sanitization reduces microbial contamination of the chromatographic bed to a minimum. MabSelect SuRe is alkali-tolerant allowing the use of NaOH as sanitizing agent. NaOH(0.1–0.5 M NaOH) is very effective for inactivating viruses, bacteria, yeasts, and endotoxins. In addition, NaOH is inexpensive compared with other sanitizing agents.
• Pack size: 25 mL
• Maximum flow velocity: 500 cm/h