MabSelect SuRe LX protein A resin

• Cat#No#: Ma-330C
• Product Overview: MabSelect SuRe LX is a protein A affinity resin with very high dynamic binding capacity at extended residence times. The chromatography resin is developed for processing of monoclonal antibodies at high titers.
  Novel, alkali-tolerant rProtein A ligand withstands rigorous clean-in-place and sanitization procedures with 0.1 to 0.5 M NaOH.
  High dynamic binding capacity (DBC) for high titer cultures reduces process time and amount of resin used.
  Fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Description: MabSelect SuRe LX is an alkali-stabilized, protein A-derived affinity medium with a binding capacity for monoclonal antibodies (MAbs) exceeding that of MabSelect SuRe at longer residence times. As an example, at 6 min residence time, the dynamic binding capacity of MabSelect SuRe LX for human IgG is approximately 60 g/l. This special combination of high binding capacity plus alkaline stability gives manufacturers of MAbs many opportunities to improve process economics and product quality.
  High dynamic binding capacity (DBC) helps users process feed from high-expression cell cultures with increased antibody titers in shorter time or with smaller chromatography unit operations. Enhanced alkaline stability means that regular cleaning-in-place (CIP) can be performed with cost-effective agents such as sodium hydroxide (NaOH) to prolong the medium's working lifetime.
• Characteristic: Outstanding binding capacity, for example, approx. 60 g/l medium for human IgG at 6 min residence time.
  Quick, efficient processing of large volumes of high-titer bioreactor feeds.
  Higher density eluates increase operating flexibility and allow smaller unit operations.
  Effective CIP with 0.1 M NaOH over hundreds of purification cycles improves process economy.
  Enhanced protease resistance of the protein A ligand reduces leakage.
  Generic elution conditions for different MAbs enable platform purifications.
• Applications: Alkali tolerance, high capacity and low ligand leakage in combination with the rigid base matrix makes MabSelect SuRe LX an excellent choice for purification of monoclonal antibodies for clinical applications.
• Ligand Coupling Method: Single-point attachment
• Column: HiScreen MabSelect SuRe, 4.7 ml
• Matrix: Highly cross-linked agarose
• Average particle size: ~85 µm
• Ligand: Alkali-stabilized Protein A-derived (E. coli)
• Coupling chemistry: Epoxy
• Dynamic binding capacity: Approximately 60 mg human IgG/mL resin.
• Recommended flow rate: 0.5 ml/min
• Chemical stability: Stable in all aqueous buffers commonly used in Protein A Chromatography
• pH working range: 3–12
• pH CIP range: 2–13.7
• CIP stability: 0.1 to 0.5 M NaOH
• Temperature stability: 2°C to 40°C
• Storage: 2 to 8°C, 20% Ethanol
• Shipping: 20% ethanol
• Cleaning-in-place: 1.Wash the column with 3 CV of binding buffer.
  2.Wash with at least 2 CV of 0.1–0.5 M NaOH. Contact time 10–15 minutes.
  3.Wash immediately with at least 5 CV of sterile and filtered binding buffer at pH 7–8.
• Sanitization: Sanitization reduces microbial contamination of the chromatographic bed to a minimum. MabSelect SuRe LX is alkalitolerant allowing the use of NaOH as sanitizing agent. NaOH is very effective for inactivating viruses, bacteria, yeasts, and endotoxins. In addition, NaOH is inexpensive compared with other sanitizing agents.
• Pack size: 25 mL
• Maximum flow velocity: 500 cm/h
• Wash buffer: 10 mM phosphate, 27 mM KCl, 140 mM NaCl, pH 7.4;50 mM acetate buffer, pH 6.0.