MabSelect SuRe pcc protein A resin

• Cat#No#: Ma-331C
• Product Overview: MabSelect SuRe pcc protein A resin is an affinity chromatography resin for purification of monoclonal antibody and Fc-fusion proteins. The high resin capacity at short residence times makes it an excellent choice for periodic counter-current chromatography in monoclonal antibody (mAb) purification. The alkaline-stable, recombinant protein ligand allows for efficient resin cleaning.
  High dynamic binding capacity.
  High productivity through capture of large mass of monoclonal antibody in a short period of time.
  Covered by a comprehensive security of supply program, including dual sources of the agarose base matrix and protein A ligand.
• Description: MabSelect SuRe pcc affinity chromatography medium (resin) offers exceptional capacity at short residence time, making it well-suited for applications requiring fast mass transfer such as MAb capture in a continuous process.
• Characteristic: Exceptional binding capacity.
  High productivity through capture of large mass of MAbs in a short period of time.
  Highly concentrated elution pools for operating flexibility and small unit operations.
  Allows cost-effective cleaning with 0.1–0.5 M NaOH over hundreds of purification cycles.
  High ligand stability reduces ligand leakage.
  Generic elution conditions for different MAbs, enabling platform purifications.
• Applications: The MabSelect family, comprising MabSelect, MabSelect Xtra, MabSelect SuRe, and MabSelect SuRe LX chromatography media, was developed for high-capacity MAb capture at process scale. To enable the high mass transfer required for continuous chromatography, MabSelect SuRe pcc was further developed from MabSelect SuRe LX. The high dynamic binding capacity (DBC) for MAbs at short residence time contributes to increased productivity by reducing total time required for column load operations. Comparative studies with MabSelect SuRe LX show that higher capacities can be achieved at shorter residence times with MabSelect SuRe pcc.
• Sample preparation: Chinese hamster ovary (CHO) cell culture, containing 4.0 g mAb/L, was harvested by centrifugation followed by filtration using an ULTA Pure HC 0.6/0.2 µm filter.
• Ligand Coupling Method: Epoxy activation
• Matrix: Highly cross-linked agarose
• Average particle size: ~50 µm
• Ligand: Alkali-stabilized Protein A-derived (E. coli)
• Dynamic binding capacity: ~ 60 mg human IgG/mL resin at 2.4 min residence time.
• Chemical stability: Stable in all aqueous buffers commonly used in Protein A Chromatography.
• pH working range: 3–12
• pH CIP range: 2–13.7
• CIP stability: 0.1–0.5 M NaOH
• Storage: 2 to 8°C, 20% Ethanol
• Shipping: 20% ethanol
• Cleaning-in-place: 0.1 M sodium hydroxide (5 min)
• Pack size: 25 mL