MabSelect Xtra antibody purification chromatography resin

• Cat#No#: Ma-333C
• Product Overview: MabSelect Xtra, with extra high dynamic binding capacity, is a protein A resin developed to meet the demands of increasing levels of expression in monoclonal antibody feedstocks.
  Higher dynamic binding capacity than currently available Protein A chromatography resins.
  Improved process economics through reduced raw materials costs and/or reduced number of cycles.
  High purity capture due to high ligand selectivity and matrix hydrophilicity.
  Slightly lower pressure/flow properties compared to MabSelect SuRe and MabSelect.
  Resin fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Description: MabSelect Xtra is a recombinant protein A-based affinity medium engineered to give an exceptionally high dynamic binding capacity for monoclonal antibodies. In addition, MabSelect Xtra is optimized for Fc-fusion proteins. Higher capacity translates directly to lower cost of production.
• Characteristic: High dynamic binding capacity and suitable for highexpression feedstocks.
  Improved process economics through reduced raw materials costs and/or reduced number of cycles.
  Very low, unspecific binding due to high ligand selectivity and matrix hydrophilicity.
  High capacity for many Fc-fusion proteins.
• Ligand Coupling Method: Epoxy activation
• Matrix: Highly cross-linked agarose
• Average particle size: ~75 µm
• Ligand: Recombinant protein A (E.coli)
• Coupling chemistry: Epoxy
• Dynamic binding capacity: Approx. 40 mg human lgG/mL at 2.4 min residence time
• Recommended flow rate: 100–300 cm/h
• Chemical stability: Stable in all aqueous buffers commonly used in protein A chromatography: 0.1 M 20% sodium citrate/HCl (pH 3), 6 M Gua-HCl, 6 M urea, 20% ethanol, 2% benzyl alcohol.
• pH working range: 3–10
• CIP stability: 2–12
• Temperature stability: 2°C–40°C
• Storage: 2 to 8°C, 20% Ethanol
• Shipping: 20% ethanol
• Cleaning-in-place: Wash with 2 column volumes of 100 mM 1-Thioglycerol pH 8.5 followed by CIP with 2 column volumes of 15 mM NaOH. Use a contact time of 15 min for each step. Wash immediately with at least 5 column volumes of sterile filtered binding buffer at pH 7–8. Reversed flow direction.
• Sanitization: Sanitization reduces microbial contamination of the bed to a minimum. Equilibrate the column with a solution of 2% hibitane digluconate and 20% ethanol. Allow to stand for 6 hours, then wash with at least 5 column volumes of sterile binding buffer. Equilibrate the column with a solution of 0.1 M acetic acid and 20% ethanol. Allow to stand for 1 hour, then wash with at least 5 column volumes of sterile binding buffer. Equilibrate the column with 70% ethanol. Allow to stand for 12 hours, then wash with at least 5 column volumes of sterile binding buffer.
• Pack size: 200 mL
• Maximum flow velocity: 300 cm/h