MacroCap SP

• Cat#No#: Ma-451C
• Product Overview: MacroCap SP is a cation exchanger designed for the purification of large biomolecules such as polyethylene glycol (PEG)-modified proteins (i.e., PEGylated proteins) that are intended for use as biopharmaceuticals.
• Description: MacroCap SP is a cation exchanger designed to separate PEGylated proteins and other large biomolecules. It allows separation of mono- from oligo- and non-PEGylated proteins with high selectivity under high load conditions. MacroCap SP is based on acrylamide-dextran copolymer resins with mass transfer properties suitable for large biomolecules. The base matrix is highly porous, which gives high available surface area for adsorption of PEGylated proteins. The large pore size also makes MacroCap SP suitable for binding other large proteins, e.g. IgM.
• Characteristic: Strong cation exchanger for PEGylated proteins and other large biomolecules.
  High purity and yield of PEGylated proteins at high sample loads.
  Highly porous base matrix makes extensive surface area available for adsorption of large molecules.
• Metal ion capacity: 0.10-0.13 mmol H+/ml medium
• Matrix: Acrylamide-dextran copolymer
• Ionic Exchanger Type: Strong cation exchanger
• Average particle size: ~50 µm
• Ligand: SO3-
• Recommended flow rate: < 120 cm/h in BPG 300, 20-cm bed height or 70 cm/h in BPG 300, 30-cm bed height using process buffers with the same viscosity as water at v 3 bar (0.3 MPa).
• Chemical stability: Commonly used aqueous buffers, 0.5 M NaOH, 0.1 M citric acid, 25% ethanol, 30% propanol, 30% methanol, 50% ethylene glycol, 1% Tween-20, and 1% SDS.
• pH working range: 4–11
• CIP stability: 2–13
• Temperature stability: 4°C to 30°C
• Storage: 4 to 30°C 0.2 M Sodium Acetate in 20% Ethanol
• Cleaning-in-place: Precipitated, hydrophobically bound proteins or lipoproteins: Wash with 0.5 M NaOH at 40 cm/h with reversed flow direction. Contact time 1 to 2 h, dependent on feed. Apply a CIP-cycle using NaOH pH 13 followed by an acidic solution pH 2. It is recommendable to wash with water in between the basic and acidic solutions.
  Ionically bound proteins: Wash with 0.5 to 2 column volumes of 0.5 to 2 M NaCl with reversed flow direction. Contact time 10 to 15 min.
  Lipids and very hydrophobic proteins: Wash with 2 to 4 column volumes of 25% ethanol or 30% propanol with reversed flow direction. Contact time 1 to 2 h, dependent on feed. Alternatively, wash with 2 to 4 column volumes of 0.1% nonionic detergent with reversed flow direction. Contact time 1 to 2 h, dependent on feed.
• Sanitization: To reduce microbial contamination in the packed column, sanitize with 0.5 M NaOH with a contact time of 1 h. The CIP protocol for precipitated, hydrophobic bound proteins or lipoproteins removes bound contaminants and sanitizes the medium effectively.
• Pack size: 100 mL
• BioProcess resin: Yes