MBPTrap HP

• Cat#No#: MB-114P
• Product Overview: MBPTrap HP columns prepacked with Dextrin Sepharose High Performance provide convenient, high binding capacity, affinity purification of recombinant proteins tagged with maltose binding protein (MBP).
• Description: Dextrin Sepharose High Performance has high binding capacity for MBP-tagged proteins. This robust and stable Sepharose High Performance based resin enables elution of target protein in narrow peaks, which minimizes the need for further concentration steps.
• Characteristic: Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes.
  Compatible with commonly used buffers and easily regenerated with 0.5 M NaOH.
  Physiological conditions and mild elution preserve the activity of the target protein.
  Convenient, time-saving operation and reproducible results.
• Maximum operating pressure: 5 bar [0.5 MPa] (70 psi)
• Matrix: Rigid highly cross-linked 6% agarose
• Average particle size: 34 μm
• Ligand: Dextrin
• Dynamic binding capacity: Approx. 7 mg MBP2*-paramyosin Sal/ml medium (Mr ~70 000, multimer in solution) Approx. 16 mg MBP2*- galactosidase/ml medium (Mr ~158 000, multimer in solution).
• Recommended flow rate: < 4 ml/min
• Recommended column height: 25 mm
• Chemical stability: Stable in all commonly used aqueous buffers.
• pH working range: > 7
• CIP stability: 2 to 13
• Storage: 2 to 8°C, 20% Ethanol
• Pack size: 5 × 1 mL
• Dimensions: 7 × 25 mm
• Column volume: 1 ml
• Column i.d.: 7 mm
• Column hardware pressure limit: 5 bar (0.5 MPa)