Mono Q 5

• Cat#No#: Mo-243P
• Product Overview: Mono Q columns are strong anion exchange chromatography columns for protein analysis or small scale, high resolution polishing of proteins.
• Description: Mono Q 5/50 GL and Mono S 5/50 GL are Tricorn high performance columns. The columns are pre-packed glass columns for high performance ion exchange chromatography of proteins, peptides, polynucleotides and other biomolecules.
  The columns are supplied with two union M6 female/1/16” male for connection to FPLC System, two fingertight connector 1/16” for connecting 1/16” tubing to column and ÄKTA, two stop plugs 1/16” male to seal the column (attached to column when delivered) and instruction.
• Form: Rigid, spherical, porous monodisperse
• Characteristic: Packed with MonoBeads Q strong anion exchange chromatography resin.
  Monodisperse 10 µm porous beads provide high resolution, reproducibility, and durability.
  Tricorn column format, compatible with systems and other high-performance liquid chromatography (LC) systems.
  Greater loading capacity but slightly lower resolution than with MiniBeads columns.
• Maximum operating pressure: 40 bar [4 MPa] (580 psi)
• Metal ion capacity: 0.27–0.37 mmol Cl- /ml medium
• Matrix: Polystyrene/divinyl benzene
• Ionic Exchanger Type: Strong anion
• Particle Size: 10 µm
• Dynamic binding capacity: 50 mg
• Recommended flow rate: 0.5-3.0 ml/min
• Recommended column height: 50 mm
• pH working range: 2 to 12
• CIP stability: 1 to 14
• Storage: 4 to 30°C, 20% Ethanol
• Cleaning-in-place: 1. Wash with 2 column volumes (CV) of 2 M NaCl.
  2. Wash with 4 CV of 1 M NaOH.
  3. Wash with at least 2 CV of 2 M NaCl.
  4. Rinse with at least 2CV of distilled water until the UV-baseline and the eluent pH are stable.
  5. Wash with at least 4 CV of start buffer or storage buffer until pH and conductivity values have reached the required values.
• Maximum flow velocity: 3 ml/min
• Dimensions: 5 × 50 mm
• Column volume: 1 mL
• Column i.d.: 5 mm
• Functional group: -CH2 -N+ (CH3 )3