This enzyme facilitates high-specificity cleavage of peptide bonds at the carboxyl side of lysine (Lys) and arginine (Arg). To prevent autolysis—which typically generates fragments that compromise HPLC peptide mapping and protein sequencing—this porcine-derived trypsin is modified via reductive methylation. This stabilization ensures the protease remains resistant to self-digestion, maintaining superior enzymatic activity and reliability compared to non-modified alternatives.
Physical Appearance
Lyophilized powder
Molecular Weight
23 kDa
Resuspension Buffer
1 mM HCl is used as the Trypsin Resuspension Buffer.
Storage
Keep lyophilized form at –20 °C; once reconstituted, store at –80 °C.
Shelf life
Store at -20 ℃ for up to 24 months.
Stability
Peak activity for modified trypsin occurs between pH 7.0 and 9.0, while it undergoes reversible inactivation at pH 4.0.
Usage Notes
To optimize performance, reconstitute trypsin using the supplied buffer and pre-incubate at 30 °C for 15 minutes. Reconstituted aliquots should be thawed at ambient temperature and kept on ice until use. A protease-to-substrate ratio of 1:100 to 1:25 (w/w) is suggested for MS identification. Following thorough mixing, incubate the reaction at 37 °C for 4 hours.
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