Ion exchange chromatography (IEC) is a highly effective method for biomolecule separation. Traditional IEC requires low salt concentrations in samples. Multimodal ion exchange media (MMC) introduce aromatic rings on ligands, improving salt tolerance and eliminating the need for desalting high-salt samples, expanding IEC applications. MMCs comprise: (1) crosslinked porous, hydrophilic, chemically stable matrix; (2) functional groups with both charged and hydrophobic parts; (3) counter-ions that reversibly bind charged functional groups. MMC highly cross-linked agarose (Fast Flow) is a weak cation exchange medium with phenyl-carboxyl groups coupled to high-flow agarose beads.
Matrix
6% Highly cross-linked agarose
Appearance
White paste, may separate upon standing
Average particle size
90 μm
Ligand
Multimodal weak cationic groups
Capacity
33-48 μmol H+ / mL packed resin
Coupling chemistry
Multimodal functionalization (phenyl-carboxyl)
pH working range
4–13
pH CIP range
2–14
Chemical stability
Stable in common aqueous solutions: 1 M NaOH, 1 M acetic acid, 70% ethanol, 20% ethanol, 6 M guanidine HCl, 8 M urea
Maximum operating pressure
0.3 MPa
Maximum flow velocity
≥ 700 cm/h
Pack size
25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L; 40 L
Storage
2–30 °C in 20% ethanol or 2% benzyl alcohol
Product features
Multimodal weak cation exchange resin; multimodal ligand provides salt tolerance compared to traditional cation exchange ligands, allowing sample loading under high conductivity; high selectivity with high dynamic binding under high conductivity; widely suitable for medium purification of antibodies and recombinant proteins, removing aggregates and fragments.
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For Research or Industrial Raw Materials, Not For Personal Medical Use!