IEC is highly effective for biomolecule separation. Salt concentration in samples must be low, but multimodal ion exchange media (MMC) with phenyl-modified ligands tolerate higher salt, eliminating desalting. MMC comprises: (1) crosslinked porous, hydrophilic, chemically stable matrix— matrix is high-rigidity agarose derived from modified 6% cross-linked agarose with improved mechanical performance; (2) functional groups with charged and hydrophobic portions; (3) counter-ions reversibly binding to charged groups. multimodal cation exchange resin is a weak cation exchange medium with phenyl-carboxyl groups coupled to 40 μm high-rigidity agarose beads.
Matrix
High-rigidity agarose
Appearance
White paste, may separate upon standing
Wet Particle Size Range
36–44 μm
Ligand
Multimodal weak cationic groups
Coupling chemistry
Multimodal functionalization (phenyl-carboxyl)
pH working range
3–12
pH CIP range
2–14
Chemical stability
Stable in common aqueous solutions: 1 M NaOH, 1 M acetic acid, 6 M guanidine HCl, 8 M urea, 70% ethanol, 30% isopropanol, 20% ethanol, 2% benzyl alcohol
Maximum operating pressure
0.5 MPa
Maximum flow velocity
≥ 450 cm/h
Recommended flow rate
100–300 cm/h
Pack size
25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L; 40 L
Storage
2–30 °C in 20% ethanol or 2% benzyl alcohol
Product features
Multimodal weak cation exchange resin; multimodal ligand provides salt tolerance under high conductivity; uses high-rigidity, high-resolution agarose matrix, providing higher resolution while maintaining high throughput; widely suitable for medium and fine purification of antibodies and recombinant proteins, removing aggregates and fragments.
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For Research or Industrial Raw Materials, Not For Personal Medical Use!