Multimodal size-exclusion resin is a novel multimodal chromatography medium designed for intermediate and fine purification of viruses and other biomacromolecules. It features a dual-layer structure with a core bead and a thin outer shell. The core bead, prepared by ligand crosslinking to high-rigidity agarose, provides anion and hydrophobic interactions. The outer shell, a ligand-free agarose layer, acts as a molecular sieve. During purification, large biomolecules like viruses flow through directly while most impurities enter the bead and bind via ionic and hydrophobic interactions. Molecular weight exclusion is 700 kDa: molecules smaller than this enter the bead; larger molecules are excluded. Key features include: dual-layer structure for effective impurity binding while target proteins flow through, broad operational conditions due to target flow-through, and higher throughput and flow rates than conventional size-exclusion media.
Stable in common aqueous solutions: 1 M NaOH, 1 M acetic acid, 70% ethanol, 20% ethanol, 30% isopropanol, 6 M guanidine HCl, 0.5% Tween 80, 1 M NaOH, 30% isopropanol
Maximum flow velocity
≥ 500 cm/h, < 2 bar
Pack size
25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L; 40 L
Storage
2–30 °C in 20% ethanol or 2% benzyl alcohol
Product features
Multimodal chromatography resin combining size-exclusion and hydrophobic interaction modes; appropriate pore size selected according to target molecule size, allowing target flow-through while impurities are retained; compared to traditional SEC, significantly increases sample load and flow rate, improving production efficiency; widely suitable for capture and intermediate purification of various viruses, mRNA, and other large biomolecules.
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For Research or Industrial Raw Materials, Not For Personal Medical Use!