NHS-activated 4% cross-linked agarose is pre-activated agarose gel based on high-flow 4% cross-linked agarose with spacer arms, suitable for small molecule ligand coupling. Features: easy amide bond formation with proteins, strong chemical stability, 10-carbon spacer reduces steric hindrance, simple operation, fast flow, suitable for large-scale applications.
Matrix
4% Highly cross-linked agarose
Appearance
White paste, may separate upon standing
Wet Particle Size Range
45–165 μm
Ligand
N-Hydroxysuccinimide (NHS)
Dynamic binding capacity
16–23 μmol NHS / mL resin
Coupling chemistry
NHS ester activation
pH working range
3–13
pH CIP range
2–13
Chemical stability
Stable in all common aqueous solutions: 8 M urea, 6 M guanidine HCl, 70% ethanol
Maximum operating pressure
0.3 MPa
Maximum flow velocity
~150 cm/h
Pack size
25 mL; 100 mL; 500 mL
Storage
2–8 °C, 100% Isopropanol
Product features
Forms amide bonds with proteins; strong chemical stability; 10-carbon spacer arm reduces steric hindrance between ligand and agarose surface; easy operation; avoids toxic reagents; fast flow; suitable for large-scale applications.
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For Research or Industrial Raw Materials, Not For Personal Medical Use!