This product can be used to purify His recombinant proteins fused and expressed by various expression systems. It is based on highly cross-linked 4% agarose gel, chemically coupled with tricoordinated iminodiacetic acid (IDA) and chelated with nickel ions (Ni2+) to form a relatively stable planar quadrilateral structure. Thus, more sites can continue to coordinate with the imidazole ring on the His tag, achieving the effect of binding to the target protein.
Concentration
The gel volume accounts for 50% of the suspension volume
Particle size
45~165 μm
Binding capacity
>40 mg 6xHis protein
Volume
10 mL(5 mL gel)
Flow velocity
80~150 cm/h (0.3 MPa, 3 bar)
Balance buffer
50 mM NaH2PO4, 300 mM NaCl, pH 8.0
Rinse buffer
50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0
Washing buffer
50 mM NaH2PO4, 300 mM NaCl, 250 mM imidazole, pH 8.0
Matrix
Highly cross-linked 4% agarose microspheres
Chelate
Ni2+
Storage Buffer
1xPBS containing 20% ethanol
Storage
This product is transported at 4°C. Store at 4°C. Do not freeze the product. For long-term storage, ensure that the reagent tube is upright and the magnetic beads are immersed in the protective solution. The shelf life is 24 months.
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For Research or Industrial Raw Materials, Not For Personal Medical Use!