Ni-IDA Agarose 6FF

• Cat#No#: AC-011I
• Product Overview: Ni-IDA Agarose 6FF is designed mainly for scaling up purification of histidine-tagged proteins that are expressed in various hosts, such as E.coli, yeast, insect cells, and mammalian cells.
• Characteristic: Mechanical stable, designed for high yield, scaling up purification of histidine-tagged proteins
  Higher binding capacity
  Low leakage of nickel ions
• Matrix: 6% highly cross-linked agarose supplied as 50% slurry
• Average particle size: 45 μm - 165 μm
• Ligand: Nickel
• Dynamic binding capacity: >40 mg 6xHis-tagged protein/mL medium
• Maximum Pressure Drop: 0.3 MPa (3 bar)
• Storage: 2 - 8°C, storage buffer: 1X PBS containing 20% ethanol