| Product Information | |
| Cat#No# | AC-010I |
| Product Overview | Ni-IDA Agarose can be used to purify 6xHis-tagged proteins expressed in various hosts, such as E.coli., yeast, insect cells and mammalian cells. |
| Characteristic | Suited for routine purification of histidine-tagged proteins Higher binding capacity than Ni-NTA and Ni-Penta resins Low leakage of nickel ions |
| Matrix | 4% agarose supplied as 50% slurry |
| Average particle size | 45 μm - 165 μm |
| Ligand | Nickel |
| Dynamic binding capacity | >40 mg 6xHis-tagged protein/mL medium |
| Recommended flow rate | Gravity flow, <75 cm/hour |
| Maximum Pressure Drop | 0.1 MPa (1 bar) |
| Storage | 2 - 8°C, storage buffer: 1X PBS containing 20% ethanol |
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