His-tagged protein agarose purification resin is based on a highly cross-linked 6% agarose gel, chemically coupled with tetracoordinated nitrotriacetic acid (NTA) as a ligand, and chelated with nickel ions (Ni2) to form a very stable octahedral structure, with nickel ions in the center of the octahedron, which can protect nickel ions from the attack of small molecules, is more stable, and can withstand harsh conditions such as reducing agents, denaturants or couplants at a certain concentration. This product enables purification of the protein of interest at relatively high flow rates.
Particle size
45-165 µm
Binding capacity
> 40 mg of 6×His-tagged fusion protein/mL matrix
Matrix
Highly cross-linked 6% agarose gel
Storage Buffer
1× PBS with 20% ethanol
Storage
Store at 4°C (avoid cryopreservation) for 2 years.