Ni-Penta Agarose 6 Fast Flow, Chemical Stable

• Cat#No#: AC-001I
• Product Overview: Chemical-Tolerant Ni-Penta Agarose 6FF is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity.
  The nickel ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous, chemical-stable structure. This characteristic nickel ion bonding on the Ni-Penta resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins. In particular, Ni-Penta Agarose 6FF is well suited for direct capture of secreted polyHis-tagged proteins from the cell culture supernatant of eukaryotic cells, including CHO cells, insect High Five cells* and Sf9 cells.
  Highly cross-linked 6% agarose as the base matrix support offers excellent mechanical stability for automated, scale up purification of histidine-tagged proteins.
  * High Five™ is a trade mark of Thermo Fisher Scientific.
• Characteristic: EDTA-resistant
  High selectivity for high purity
  Retained high 6xHis affinity in various buffers
  Recharge-free with minimal nickel ion leakage
  Requires less imidazole
  NaOH-tolerant, allowing CIP with 1 M NaOH
  Versatile, ready-to-use with convenient formats
• Applications: Purification of secreted His-tagged proteins directly from eukaryotic cell culture supernatants
  Purification of His-tagged proteins that require presence of EDTA, DTT, β-ME, and other agents for maximum protein stability and integrity
  Repetitive uses of the column that require clean-in-place with NaOH but no nickel recharge
  Restricted imidazole amount in the elution buffer due to the requirements in downstream applications or the target protein's sensitivity to imidazole
• Metal ion capacity: > 50 µmol Ni2+ / ml medium
• Matrix: Highly cross-linked 6% agarose resin supplied as 50% slurry
• Average particle size: 45 μm - 165 μm
• Ligand: Nickel
• Dynamic binding capacity: >10 mg 6 X His-tagged protein/mL medium
• Maximum Pressure Drop: 0.3 MPa (3 bar)
• Chemical stability: 0.01 M HCl ,0.01 M NaOH, for ≥ 1 week;
  10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours
  500 mM imidazole，100 mM EDTA, for ≥ 2 hours
  30% isopropanol for ≥ 20 mins
• Storage: 2 - 8°C, storage buffer: 1X PBS containing 20% ethanol
• Shipping: Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze.