Cat# | Product Name | Size | Price | Qty | Inquiry |
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AC-003I | Ni-Penta Agarose 6 Fast Flow, Chemical Stable, Prepacked Cartridge | 5x1ml | Inquiry | ||
1x5ml | Inquiry | ||||
5x5ml | Inquiry |
Product Information | |
Cat#No# | AC-003I |
Product Overview | Chemical-Tolerant Ni-Penta Agarose 6FF is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity. The nickel ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous, chemical-stable structure. This characteristic nickel ion bonding on the Ni-Penta resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins. In particular, Ni-Penta Agarose 6FF is well suited for direct capture of secreted polyHis-tagged proteins from the cell culture supernatant of eukaryotic cells, including CHO cells, insect High Five cells* and Sf9 cells. Ni-Penta Agarose 6 FF Prepacked Cartridge are packed with 1 ml and 5 ml of Ni-Penta Agarose 6 Fast Flow. The columns have standard interface that can be adapted to most commonly used chromatography systems, such as ÄKTA. |
Characteristic | EDTA-resistant High selectivity for high purity Retained high 6xHis affinity in various buffers Recharge-free with minimal nickel ion leakage Requires less imidazole NaOH-tolerant, allowing CIP with 1 M NaOH Ready-to-use, convenient formats: 1 mL and 5 mL |
Applications | Purification of secreted His-tagged proteins directly from eukaryotic cell culture supernatants Purification of His-tagged proteins that require presence of EDTA, DTT, β-ME, and other agents for maximum protein stability and integrity Repetitive uses of the column that require clean-in-place with NaOH but no nickel recharge Restricted imidazole amount in the elution buffer due to the requirements in downstream applications or the target protein's sensitivity to imidazole |
Matrix | Highly cross-linked 6% agarose resin |
Average particle size | 45 μm - 165 μm |
Ligand | Nickel |
Dynamic binding capacity | >10 mg 6XHis-tagged protein/mL medium |
Maximum Pressure Drop | 0.3 MPa (3 bar) |
Chemical stability | 0.01 M HCl ,0.01 M NaOH, for ≥ 1 week; 10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours 500 mM imidazole,100 mM EDTA, for ≥ 2 hours 30% isopropanol for ≥ 20 mins |
Storage | 2 - 8°C, storage buffer: 1X PBS containing 20% ethanol |
Download Datasheet | Download SDS |
For Research or Industrial Raw Materials, Not For Personal Medical Use!
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