Product Information | |
Cat#No# | AC-003I |
Product Overview | Chemical-Tolerant Ni-Penta Agarose 6FF is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity. The nickel ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous, chemical-stable structure. This characteristic nickel ion bonding on the Ni-Penta resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins. In particular, Ni-Penta Agarose 6FF is well suited for direct capture of secreted polyHis-tagged proteins from the cell culture supernatant of eukaryotic cells, including CHO cells, insect High Five cells* and Sf9 cells. Ni-Penta Agarose 6 FF Prepacked Cartridge are packed with 1 ml and 5 ml of Ni-Penta Agarose 6 Fast Flow. The columns have standard interface that can be adapted to most commonly used chromatography systems, such as ÄKTA. |
Characteristic | EDTA-resistant High selectivity for high purity Retained high 6xHis affinity in various buffers Recharge-free with minimal nickel ion leakage Requires less imidazole NaOH-tolerant, allowing CIP with 1 M NaOH Ready-to-use, convenient formats: 1 mL and 5 mL |
Applications | Purification of secreted His-tagged proteins directly from eukaryotic cell culture supernatants Purification of His-tagged proteins that require presence of EDTA, DTT, β-ME, and other agents for maximum protein stability and integrity Repetitive uses of the column that require clean-in-place with NaOH but no nickel recharge Restricted imidazole amount in the elution buffer due to the requirements in downstream applications or the target protein's sensitivity to imidazole |
Matrix | Highly cross-linked 6% agarose resin |
Average particle size | 45 μm - 165 μm |
Ligand | Nickel |
Dynamic binding capacity | >10 mg 6XHis-tagged protein/mL medium |
Maximum Pressure Drop | 0.3 MPa (3 bar) |
Chemical stability | 0.01 M HCl ,0.01 M NaOH, for ≥ 1 week; 10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours 500 mM imidazole,100 mM EDTA, for ≥ 2 hours 30% isopropanol for ≥ 20 mins |
Storage | 2 - 8°C, storage buffer: 1X PBS containing 20% ethanol |
Download Datasheet |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Online Inquiry