Product Information | |
Cat#No# | AC-002I |
Product Overview | Chemical-Tolerant Ni-Penta Agarose is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions, designed mainly for purification of low abundant proteins or challenging proteins in conditions where EDTA, DTT, β-ME, and other stabilizing agents are necessary for maintaining protein stability and integrity. The nickel ion binds very strongly to the matrix support in complex with a proprietary pentadentate chelating ligand, which immobilizes the nickel ion through 5 sites to form a homogeneous, chemical-stable structure. This characteristic nickel ion bonding on the Ni-Penta resin provides excellent resistance to EDTA, NaOH, β-ME, and other reagents that are restricted in the purification protocol using conventional NTA- and IDA- functionalized resins. |
Characteristic | EDTA-resistant High selectivity for high purity Retained high 6xHis affinity in various buffers Well suited for direct capture of secreted polyHis-tagged proteins from eukaryotic cell culture supernatant Recharge-free with minimal nickel ion leakage Requires less imidazole NaOH-tolerant Versatile, ready-to-use with convenient formats |
Applications | Purification of secreted His-tagged proteins directly from eukaryotic cell culture supernatants Purification of His-tagged proteins that require presence of EDTA, DTT, β-ME, and other agents for maximum protein stability and integrity Repetitive uses of the column that require clean-in-place with NaOH but no nickel recharge Restricted imidazole amount in the elution buffer due to the requirements in downstream applications or the target protein's sensitivity to imidazole |
Matrix | 4% agarose supplied as 50% slurry |
Average particle size | 45 μm - 165 μm |
Ligand | Nickel |
Dynamic binding capacity | >10 mg 6XHis-tagged protein/mL medium |
Recommended flow rate | Gravity flow, <75 cm/hour |
Maximum Pressure Drop | 0.1 MPa (1 bar) |
Chemical stability | 0.01 M HCl ,0.01 M NaOH, for ≥ 1 week; 10 mM EDTA, 1 M NaOH, 5 mM DTT, 5 mM TCEP, 20 mM β-ME, 6 M guanidine-HCl, for ≥ 24 hours 500 mM imidazole,100 mM EDTA, for ≥ 2 hours 30% isopropanol for ≥ 20 mins |
Storage | 2 - 8°C, storage buffer: 1X PBS containing 20% ethanol |
Shipping | Product is shipped at ambient temperature. Upon receipt store at 2 - 8°C. Do not Freeze. |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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