Nickel Chelating Resin (Fast Flow)

• Product Overview: This Ni²⁺-charged Fast Flow IMAC resin uses NTA ligands on agarose gel. It has high binding capacity, good selectivity, easy regeneration, and low cost, widely applied in biopharmaceutical and bioengineering downstream purification, particularly for His-tagged proteins.
• Matrix: 6% Highly cross-linked agarose
• Appearance: Blue-green paste, may separate upon standing
• Wet Particle Size Range: 45–165 μm
• Ligand: Nitrilotriacetic acid (NTA)
• Dynamic binding capacity: ~40 mg His-tagged protein / mL packed resin
• Coupling chemistry: Metal chelation (nitrilotriacetic acid, NTA)
• pH working range: 3–12
• pH CIP range: 2–14
• Chemical stability: 40 °C 1 week: 10 mM HCl, 0.1 M NaOH, 8 M urea, 6 M guanidine HCl
• Maximum operating pressure: 0.3 MPa
• Pack size: 25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L
• Storage: 2–30 °C in 20% ethanol or 2% benzyl alcohol
• Product features: Ni²⁺-charged NTA (nitrilotriacetic acid) chelating ligand resin.
  Rapid and efficient purification of his-tagged proteins.
  Compatible with various purification additives, high dynamic binding capacity, high elution yield, low metal residue, high selectivity, easy regeneration, suitable for scale-up.