Nickel Chelating Resin (High Performance)

• Product Overview: This Ni²⁺-charged High Performance IMAC resin uses NTA ligands on high-resolution agarose gel. It provides high resolution, high capacity, strong selectivity, easy regeneration, and low cost, ideal for downstream purification of His-tagged proteins.
• Matrix: 6% Highly cross-linked agarose
• Appearance: Blue-green paste, may separate upon standing
• Average particle size: 34 μm
• Wet Particle Size Range: 24–44 μm
• Ligand: Nitrilotriacetic acid (NTA)
• Dynamic binding capacity: ~40 mg His-tagged protein / mL packed resin
• Coupling chemistry: Metal chelation (nitrilotriacetic acid, NTA)
• pH working range: 3–12
• pH CIP range: 2–14
• Chemical stability: 40 °C 1 week: 10 mM HCl, 0.1 M NaOH, 8 M urea, 6 M guanidine HCl
• Maximum operating pressure: 0.3 MPa
• Maximum flow velocity: < 150 cm/h
• Pack size: 25 mL; 100 mL; 500 mL; 1 L; 5 L; 10 L; 20 L
• Storage: 2–30 °C in 20% ethanol or 2% benzyl alcohol
• Product features: Ni²⁺-charged NTA ligand resin.
  High-resolution purification of his-tagged proteins, compatible with additives.
  high dynamic binding capacity, high elution yield, low metal residue.