nProtein A Sepharose Fast Flow antibody purification resin

• Cat#No#: nP-338C
• Product Overview: nProtein A Sepharose 4 Fast Flow is a native protein A resin for purification of monoclonal and polyclonal antibodies.
  For the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.
  Used in routine commercial polyclonal and monoclonal antibody purification and production.
  Free from animal-derived components.
  Well suited to immunoprecipitation procedures.
  Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool. Fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Description: nProtein A Sepharose 4 Fast Flow is native protein A coupled to Sepharose 4 Fast Flow. It has nearly twice the total IgG binding capacity of Protein A Sepharose CL-4B, and is an excellent adsorbent for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale.
  nProtein A Sepharose 4 Fast Flow has been developed and tested in cooperation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.
• Characteristic: Low leakage of protein A.
  Used in large-scale FDA-approved processes.
  Manufactured without using animal-derived components.
• Applications: The most important application area for nProtein A Sepharose 4 Fast Flow is the purification of monoclonal antibodies from cell culture. High IgG capacity and high flow velocities make the resin ideal for both laboratory- and process-scale separations.
  There is a natural diversity between the different subclasses of IgG and even within subclasses. Therefore the binding and elution system must be optimized for every monoclonal antibody to be purified.
• Maximum operating pressure: < 0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20˚C using buffers with the same viscosity as water).
• Ligand Coupling Method: Cyanogen bromide activation
• Matrix: cross-linked agarose, 4%, spherical
• Average particle size: ~90 µm
• Ligand density: 6 mg Protein A/ml medium
• Dynamic binding capacity: >35 mg human IgG/mL
• Recommended flow rate: 30 to 200 cm/h
• Recommended column height: 25 cm
• Chemical stability: Stable in aqueous buffers commonly used in Protein A chromatography, 6 M guanidine-HCl, 70% ethanol, 3 M NaSCN, 0.1 M glycine (pH 3.0), 2% benzyl alcohol or 20 % ethanol.
• pH working range: 3–9
• CIP stability: 2–10
• Temperature stability: 4°C to 40°C
• Storage: 2 to 8°C, 20% Ethanol
• Cleaning-in-place: As cleaning protocol, 6 M guanidine hydrochloride can be used. Phosphoric acid (100 mM) has also been used for cleaning. To remove hydrophobically-bound substances, a solution of non-ionic detergent or ethanol is recommended.
• Sanitization: Wash the packed column with 2% hibitane/20% ethanol or 70% ethanol.
• Pack size: 5 mL
• Maximum flow velocity: 200 cm/h
• Dimensions: 5 cm