nProtein A Sepharose Fast Flow resin

• Cat#No#: nP-435C
• Product Overview: nProtein A Sepharose 4 Fast Flow is a native protein A resin for purification of monoclonal and polyclonal antibodies.
  For the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.
  Used in routine commercial polyclonal and monoclonal antibody purification and production.
  Free from animal-derived components.
  Well suited to immunoprecipitation procedures.
  Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cell-derived impurities in the elution pool. Fulfills industrial demands for security of supply, robust performance, and regulatory support.
• Description: nProtein A Sepharose 4 Fast Flow is native protein A coupled to Sepharose 4 Fast Flow. It has nearly twice the total IgG binding capacity of Protein A Sepharose CL-4B, and is an excellent adsorbent for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale.
• Characteristic: Low leakage of protein A.
  Used in large-scale FDA-approved processes.
  Manufactured without using animal-derived components.
• Maximum operating pressure: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20⁰C using buffers with the same viscosity as water).
• Sample preparation: 10 mL filtered cell culture supernatant, equilibrated to buffer A by addition of dry buffers salts and titration with NaOH
• Ligand Coupling Method: Cyanogen bromide activation
• Matrix: cross-linked agarose, 4%, spherical
• Average particle size: ~90 µm
• Ligand: Native Staphylococcal protein A
• Ligand density: 6 mg Protein A/ml medium
• Dynamic binding capacity: >35 mg human IgG/mL
• Recommended flow rate: 150-250 cm/h
• Recommended column height: 25 cm
• Chemical stability: Stable in aqueous buffers commonly used in Protein A chromatography, 6 M guanidine-HCl, 70% ethanol, 3 M NaSCN, 0.1 M glycine (pH 3.0), 2% benzyl alcohol or 20 % ethanol.
• pH working range: 3–9
• CIP stability: 2–10
• Temperature stability: 4°C to 40°C
• Storage: 2 to 8°C, 20% Ethanol
• Cleaning-in-place: As cleaning protocol, 6 M guanidine hydrochloride can be used. Phosphoric acid (100 mM) has also been used for cleaning. To remove hydrophobically-bound substances, a solution of non-ionic detergent or ethanol is recommended.
• Sanitization: Wash the packed column with 2% hibitane/20% ethanol or 70% ethanol.
• Pack size: 5 mL
• BioProcess resin: Yes
• Dimensions: 1×1.25 cm (1 mL)