Nuvia IMAC Resin

• Cat#No#: IMAC-001R
• Product Overview: Use high-capacity Nuvia IMAC Resin for purification of recombinant histidine-tagged and other metal binding proteins. Resin can be used for batch protocols or with gravity, low-pressure, and medium-pressure chromatography systems.
  Chelating ligand is nitrilotriacetic acid (NTA)
  Stable across a wide pH range (2–14), with high NaOH stability
  Works under native or denaturing conditions
  Can be used for single-step purification from crude samples or contaminant removal
  Nuvia IMAC Resin is compatible with denaturants (including 8 M urea and 6 M guanidine HCl), detergents, reducing agents, alcohols, chelating agents, salts, and other common protein purification reagents.
• Form: 50% slurry in 20% ethanol
• Characteristic: Chelating ligand is nitrilotriacetic acid (NTA)
  Stable across a wide pH range (2–14), with high NaOH stability
  Works under native or denaturing conditions
  Can be used for single-step purification from crude samples or contaminant removal
• Capacity: ≥ 18 µmol Cu2+/ml resin, 6x histidine-tagged proteins >40 mg/ml resin (dynamic binding capacity varies from protein to protein)
• Maximum operating pressure: 45 psi (3.1 bar)
• Average particle size: 38–53 µm (mean 50 µm)
• Ligand: Nitrilotriacetic acid (NTA)
• Recommended flow rate: <500 cm/hr at 25°C
• Temperature stability: 4–40°C
• Autoclavable: Yes (in 0.1 M sodium acetate)
• Shelf life: ≥3 years at ambient temperature in 20% ethanol
• Storage: 4°C or ambient