Octyl Sepharose 4 Fast Flow

• Cat#No#: Oc-421C
• Product Overview: Octyl Sepharose 4 Fast Flow is a well established, standard aliphatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification of larger proteins.
  Aliphatic HIC resins based on Sepharose Fast Flow base matrix derivatized via uncharged, chemically-stable ether linkages.
  The octyl derivative adds a different and complementary selectivity to the Sepharose Fast Flow HIC product family.
  Optimized for the separation of larger proteins in capture and intermediate purification steps.
  BioProcess resin supported for industrial applications and well established in approved processes.
• Description: Octyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades. This resin is based on cross-linked 4% agarose. The aliphatic ligand is immobilized to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects. Octyl Sepharose 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
• Maximum operating pressure: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20⁰C using buffers with the same viscosity as water).
• Sample preparation: The sample must be dissolved in start buffer. Alternatively the sample can be transferred to start buffer by dialysis or by buffer exchange using a HiTrap Desalting or a PD-10 Desalting column. The viscosity of the sample must not exceed that of the buffer. For normal aqueous buffer systems, this corresponds to a protein concentration of approximately 50 mg/mL.
  Before application the sample must be centrifuged or filtered through a 0.45 μm filter to remove any particulate matter.
• Matrix: cross-linked agarose, 4%, spherical
• Particle Size: 45 µm-165 µm
• Average particle size: ~90 μm
• Ligand: Octyl
• Ligand density: ~5 µmol Octyl/mL resin
• Recommended column height: 25 cm
• Chemical stability: Stable in commonly used aqueous buffers - 1.0 M NaOH, 30% isopropanol, 70% ethanol, 6 M guanidine-hydrochloride, 30% Acetonitrile, 1mM HCl.
• pH working range: 3–13
• CIP stability: 2–14
• Temperature stability: 4°C to 40°C
• Autoclavable: 20 min at 121°C in distilled water pH 7, 5 cycles.
• Storage: 4 to 30°C, 20% Ethanol
• Shipping: 20% ethanol
• Cleaning-in-place: Remove strongly bound hydrophobic proteins, lipoproteins, and lipids: Wash the column with 4 to 10 bed volumes of up to 70% ethanol or 30% isopropanol followed by 3 to 4 bed volumes of water. Apply gradients to avoid air bubble formation when using high concentrations of organic solvents.
  Remove other contaminants the following method is suggested: Wash the column with 4 bed volumes of 0.5 to 1.0 M NaOH at 40 cm/h, followed by 2 to 3 bed volumes of water.
  Alternatively, wash the column with 1 to 2 bed volumes of 0.5% nonionic detergent followed by 5 bed volumes of 70% ethanol to remove the detergent, and 3 to 4 bed volumes of water.
• Sanitization: Wash the column with 0.5 to 1.0 M NaOH at a flow velocity of approximately 40 cm/h, contact time 30 to 60 minutes.
• Pack size: 200 mL
• BioProcess resin: Yes
• Dimensions: 5 cm