Phenyl Sepharose 6 Fast Flow (High Sub)

• Cat#No#: Ph-423C
• Product Overview: Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity.
  Standard aromatic HIC resins based on Sepharose Fast Flow base matrix derivatized via uncharged, chemically-stable ether linkages. Designed for initial and intermediate purification requiring low to medium hydrophobicity.
  Two levels of substitution help find optimal selectivity and binding capacity for a given application.
  BioProcess resin supported for industrial applications and well-established in approved processes.
  The hydrophilic nature of the base matrix ensures low levels of non-specific binding.
• Description: Phenyl Sepharose 6 Fast Flow (high substitution) is resins for hydrophobic interaction chromatography (HIC). Both resins satisfy process chromatography requirements in terms of performance, stability, scaleability, and bulk availability. As part of the BioProcess range of resins, Phenyl Sepharose 6 Fast Flow (high sub) carry comprehensive technical and regulatory support for production scale applications. The resins have long track records of use in downstream processing of biopharmaceuticals.
• Characteristic: High dynamic binding capacity and stability.
  Fast Flow matrix gives high flow rates.
  Highly hydrophilic base matrix making true hydrophobic interaction chromatography possible without interfering secondary interactions influencing protein conformation or binding.
  Suitable for a wide range of applications from research to production scale.
• Maximum operating pressure: 250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20⁰C using buffers with the same viscosity as water).
• Matrix: cross-linked agarose, 6%, spherical
• Particle Size: 45 µm-165 µm
• Average particle size: ~90 µm
• Ligand: Phenyl
• Recommended column height: 25 cm
• Chemical stability: Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea.
• pH working range: 3–13
• CIP stability: 2–14
• Temperature stability: 4°C to 30°C
• Autoclavable: 20 min at 121°C in distilled water pH 7, 5 cycles.
• Storage: 4 to 30°C, 20% Ethanol
• Shipping: 20% ethanol
• Cleaning-in-place: Remove precipitated proteins: Wash the column with 4 bed volumes of 0.5 to 1.0 M NaOH solution at a flow velocity of 40 cm/h, followed by 2 to 3 bed volumes of water.
  Remove strongly bound hydrophobic proteins, lipoproteins and lipids: Wash the column with 4 to 10 bed volumes of up to 70% ethanol or 30% isopropanol. Alternatively, wash the column with detergent in a basic or acidic solution. Wash at a flow velocity of 40 cm/h. Residual detergent can be removed by washing with 5 bed volumes of 70% ethanol.
• Sanitization: Sanitization using NaOH reduces microbial contamination of the resin bed to a minimum, without dismantling the column. The CIP procedures recommended above also sanitize Phenyl Sepharose Fast Flow resins effectively. A concentration of 0.5 to 1.0 M NaOH with a contact time of 30 to 60 min has proved effective for most microbial contaminations.
• Pack size: 1 L
• BioProcess resin: Yes
• Dimensions: 5 cm