Phenyl Sepharose 6 Fast Flow (Low Sub)

• Cat#No#: Ph-424C
• Product Overview: Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity.
  Standard aromatic HIC resins based on Sepharose Fast Flow base matrix derivatized via uncharged, chemically-stable ether linkages. Designed for initial and intermediate purification requiring low to medium hydrophobicity.
  Two levels of substitution help find optimal selectivity and binding capacity for a given application.
  BioProcess resin supported for industrial applications and well-established in approved processes.
  The hydrophilic nature of the base matrix ensures low levels of non-specific binding.
• Description: Phenyl Sepharose 6 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades. Phenyl Sepharose 6 Fast Flow is composed of cross-linked, 6% agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages. Two levels of ligand substitution degree help to find the optimal selectivity and binding capacity for a given application.
• Characteristic: High dynamic binding capacity and stability.
  Fast Flow matrix gives high flow rates.
  Highly hydrophilic base matrix making true hydrophobic interaction chromatography possible without interfering secondary interactions influencing protein conformation or binding.
  Suitable for a wide range of applications from research to production scale.
• Maximum operating pressure: 250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20⁰C using buffers with the same viscosity as water).
• Matrix: cross-linked agarose, 6%, spherical
• Particle Size: 45 µm-165 µm
• Average particle size: ~90 µm2022/5/16~90 µm
• Ligand: Phenyl
• Ligand density: ~25 µmol phenyl/mL resin
• Recommended column height: 25 cm
• Chemical stability: Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea.
• pH working range: 3–13
• CIP stability: 2–14
• Temperature stability: 4°C to 30°C
• Autoclavable: 20 min at 121°C in distilled water pH 7, 5 cycles.
• Storage: 4 to 30°C, 20% Ethanol
• Shipping: 20% ethanol
• Cleaning-in-place: Remove precipitated proteins: Wash the column with 4 bed volumes of 0.5 to 1.0 M NaOH solution at a flow velocity of 40 cm/h, followed by 2 to 3 bed volumes of water.
  remove strongly bound hydrophobic proteins, lipoproteins and lipids: Wash the column with 4 to 10 bed volumes of up to 70% ethanol or 30% isopropanol. Alternatively, wash the column with detergent in a basic or acidic solution. Wash at a flow velocity of 40 cm/h. Residual detergent can be removed by washing with 5 bed volumes of 70% ethanol.
• Sanitization: For inactivation of microbial contaminants, equilibrate the column with 0.5 to 1.0 M NaOH at a flow velocity of approximately 40 cm/h, contact time 30 to 60 minutes.
  Wash the column thoroughly with running buffer after sanitization.
• Pack size: 25 mL
• BioProcess resin: Yes
• Dimensions: 5 cm