Phenyl Sepharose CL-4B

• Cat#No#: Ph-425C
• Product Overview: Phenyl-Sepharose CL-4B is a well-proven aromatic resin for hydrofobic interaction chromatography (HIC). Cross-linked agarose, 4%, spherical. Aromatic ligand.
• Description: Phenyl Sepharose CL-4B is a separation resin for hydrophobic interaction chromatography (HIC). Substances are separated on the basis of their different hydrophobicity.
  The phenyl group is covalently coupled to a cross-linked 4% agarose matrix by ether linkage, giving a hydrophobic resin with minimal leakage and no ionic properties.
• Matrix: Cross-linked agarose, 4%, spherical
• Particle Size: 45 μm-165 μm
• Average particle size: ~90 μm
• Ligand: Phenyl
• Ligand density: 40 μlmole/mL drained medium
• Dynamic binding capacity: HSA (Mr 68 000) 15-20 mg/mL drained resin, ß-lactoglobulin (Mr 18 000) 3-5 mg/mL drained resin.
• Recommended flow rate: < 150 cm/h
• Chemical stability: Stable in commonly used aqueous buffers and additives such as 8 M urea and 6 M guanidine hydrochloride.
• Physical stability: Negligible volume variation due to changes in pH or ionic strength.
• pH working range: 3–12
• CIP stability: 4–12
• Autoclavable: 121°C for 30 min
• Storage: 4 to 30°C, 20% Ethanol or 0.1 M NaOH.
• Cleaning-in-place: Remove precipitated proteins, tightly bound proteins, lipids and lipoproteins by washing the column, in reversed flow direction, with 2 to 3 bed volumes of 0.01 M NaOH. Stop the flow and let it stand for a maximum of 4 hours.
  Wash with distilled water, at least 2 to 3 bed volumes, until the pH of the effluent is neutral. Re-equilibrate with at least 3 bed volumes of binding buffer.
  Alternatively, wash the column with 4 bed volumes of up to 70% ethanol or 30% isopropanol or with 2 bed volumes of detergent in a basic or acidic solution. Wash at a low flow velocity of approximately 40 cm/h, contact time 1 to 2 hours, reversed flow direction. When using high concentrations of organic solvents, apply increasing gradients to avoid air bubble formation.
• Sanitization: 0.5–1 M NaOH for at least 5 hours
• Pack size: 200 mL